Test ID 7AC4 7AC4, Bile Acid Synthesis, Serum
Specimen Required
Patient Preparation:
1. Patient must be fasting for at least 12 hours; fasting morning specimen is preferred.
2. Patient should not be taking bile acid sequestrants or statins.
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Centrifuge and aliquot 1 mL of serum into plastic vial.
2. Send specimen frozen.
Useful For
Screening for bile acid malabsorption in patients with irritable bowel syndrome-diarrhea (IBS-D)
Method Name
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
7AC4, Bile Acid Synthesis, SSpecimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Frozen (preferred) | 90 days | |
Refrigerated | 72 hours | ||
Ambient | 24 hours |
Reference Values
≥18 years: 2.5-63.2 ng/mL
Reference values have not been established for patients who are <18 years of age.
Day(s) Performed
Wednesday
Report Available
2 to 9 daysPerforming Laboratory

Test Classification
This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82542
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
7AC4 | 7AC4, Bile Acid Synthesis, S | 94866-1 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
65504 | 7AC4, Bile Acid Synthesis, S | 94866-1 |
Forms
If not ordering electronically, complete, print, and send Gastroenterology and Hepatology Client Test Request (T728) with the specimen