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Test ID AFH Factor H Autoantibody, Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Immediately after specimen collection, place the tube on wet ice.

2. After sample has clotted on wet ice, centrifuge at 4° C and aliquot serum into a plastic vial.

3. Freeze specimen within 30 minutes of centrifugation. Sample must be placed on dry ice if not frozen immediately.

Additional Information: If the specimen is to be shared with AHUSD / Atypical Hemolytic Uremic Syndrome Complement Panel, Serum and Plasma, only serum collected in a red-top tube is acceptable.

NOTE: If a refrigerated centrifuge is not available, it is acceptable to use a room temperature centrifuge, provided the sample is kept on ice before centrifugation, and immediately afterward, the serum aliquoted and frozen..


Useful For

Detection and quantification of antibodies to factor H

 

Monitoring patients with known factor H autoantibodies

 

Aiding in the differential diagnosis of thrombotic microangiopathy and C3 glomerulopathies

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Factor H Autoantibody, S

Specimen Type

Serum Red

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Frozen (preferred) 28 days
  Refrigerated  28 days
  Ambient  14 days

Reference Values

<15.8 U/mL

Day(s) Performed

Monday

Report Available

2 to 8 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83520

LOINC Code Information

Test ID Test Order Name Order LOINC Value
AFH Factor H Autoantibody, S 101863-9

 

Result ID Test Result Name Result LOINC Value
AFH Factor H Autoantibody, S 101863-9