Test ID AHEP Acute Hepatitis Profile, Serum
Necessary Information
Date of collection is required.
Specimen Required
Two aliquots of serum are required for testing: 0.5-mL of refrigerated serum and 2.5-mL of frozen serum
Patient Preparation: For 24 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.
Collection Container/Tube: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 3 mL
Collection Instructions:
1. Centrifuge blood collection tube per collection tube manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).
2. Aliquot 0.5 mL serum into a plastic vial labeled as HAIGM, and ship refrigerate (required).
3. Aliquot remaining 2.5 mL serum into a second plastic vial labeled as SST Serum, and ship frozen (preferred).
Useful For
Differential diagnosis of recent acute viral hepatitis
Profile Information
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
HAIGM | Hepatitis A IgM Ab, S | Yes | Yes |
HBAG | HBs Antigen, S | Yes | Yes |
HBIM | HBc IgM Ab, S | Yes | Yes |
HCVDX | HCV Ab w/Reflex to HCV PCR, S | Yes | Yes |
Testing Algorithm
If hepatitis C virus (HCV) antibody is reactive, then HCV RNA detection and quantification by real-time reverse transcription-polymerase chain reaction will be performed at an additional charge.
If hepatitis B surface antigen is reactive, then confirmation will be performed at an additional charge.
The following algorithms are available:
-Hepatitis B: Testing Algorithm for Screening, Diagnosis, and Management
Special Instructions
Method Name
HAIGM: Chemiluminescent Microparticle Immunoassay (CMIA)
HBAG, HBIM, HCVDX, HBGNT: Chemiluminescence Immunoassay (CIA)
HCVQN: Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
Reporting Name
Acute Hepatitis ProfileSpecimen Type
SerumSerum SST
Specimen Minimum Volume
2 mL (send 0.5 mL refrigerate and the remaining 1.5 mL frozen)
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated | 5 days | |
Serum SST | Frozen (preferred) | 28 days | |
Refrigerated | 5 days |
Reference Values
HEPATITIS B SURFACE ANTIGEN
Negative
HEPATITIS B SURFACE ANTIGEN CONFIRMATION
Negative
HEPATITIS B CORE IgM ANTIBODY
Negative
HEPATITIS A IgM ANTIBODY
Negative
HEPATITIS C ANTIBODY
Negative
HEPATITIS C VIRUS RNA DETECTION AND QUANTIFICATION BY REAL-TIME RT-PCR
Undetected
Interpretation depends on clinical setting. See Viral Hepatitis Serologic Profiles
Day(s) Performed
Monday through Saturday
Report Available
Same day/1 to 2 daysPerforming Laboratory

Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
80074 (if all 4 initial tests are performed)
86709 (if all 4 are not performed)
86705 (if all 4 are not performed)
87340 (if all 4 are not performed)
86803 (if all 4 are not performed)
87522 (if appropriate)
87341 (if appropriate)
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
AHEP | Acute Hepatitis Profile | 24363-4 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
HCVA4 | HCV Ab, S | 40726-2 |
HBIM | HBc IgM Ab, S | 24113-3 |
H_BAG | HBs Antigen, S | 5196-1 |
HAIGM | Hepatitis A IgM Ab, S | 13950-1 |
Reflex Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
HBGNT | HBs Antigen Confirmation, S | No | No |
HCVQN | HCV RNA Detect/Quant, S | Yes | No |
Forms
If not ordering electronically, complete, print, and send 1 of the following: