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HelpTest ID AHPV Anal Pap Human Papillomavirus (HPV) with Genotyping, High-Risk Types, PCR, Varies
Necessary Information
Specimen source collection date, and patient identifiers are required.
Specimen Required
Submit one of the following specimens:
Preferred
Specimen Type: Anus or rectum
Supplies: ThinPrep Media with Spatula and Brush Kit (T434)
Collection Container/Tube: Brush
Submission Container/Tube: ThinPrep
Specimen Volume: A minimum of 20 mL or entire collection
Collection Instructions:
1. Visualize the anal opening by retracting the buttocks.
2. Insert brush past internal anal sphincter until it abuts the distal rectal wall (4-5cm).
3. Rotate the brush 360 degrees and maintain lateral pressure on brush against the walls of the anus. The brush should bow slightly due to the pressure.
4. While rotating, slowly withdraw the brush.
5. Rotate at least 10 times while withdrawing the brush. This should take 20 to 30 seconds.
6. Immediately place brush in the ThinPrep solution and swish at least 20 to 30 seconds.
7. Dispose of the brush.
8. Tighten the cap so that the torque line on the cap passes the torque line on the vial.
9. Bag ThinPrep specimens individually as they tend to leak during transport.
10. Place labels on the vial and on the biohazard bag.
Acceptable
Specimen Type: Anus or rectum
Supplies: PreservCyt Vial (T536)
Collection Container/Tube: Dacron/polyester swab
Submission Container/Tube: PreservCyt solution vial
Specimen Volume: A minimum of 20 mL or entire collection
Collection Instructions:
1. Visualize the anal opening by retracting the buttocks.
2. Insert Dacron or polyester swab past internal anal sphincter until it abuts the distal rectal wall (4-5cm).
3. Rotate the swab 360 degrees and maintain lateral pressure on swab against the walls of the anus. The swab should bow slightly due to the pressure.
4. While rotating, slowly withdraw the swab.
5. Rotate at least 10 times while withdrawing the swab. This should take 20 to 30 seconds.
6. Immediately place swab in the ThinPrep solution. Swish at least 20 to 30 seconds in the ThinPrep solution.
7. Dispose of the swab.
8. Tighten the cap so that the torque line on the cap passes the torque line on the vial.
9. Bag ThinPrep specimens individually as they tend to leak during transport.
10. Place labels on the vial and on the biohazard bag.
Useful For
Detection of high-risk (HR) genotypes associated with the development of anal cancer
Individual genotyping of human papillomavirus (HPV)-16 and HPV-18 if present
May aid in triaging men and women with positive HR HPV but negative anal Papanicolaou (Pap) smear test results
This test is not intended for use in medical-legal applications.
Method Name
Real-Time Polymerase Chain Reaction (PCR)
Reporting Name
HPV Anal Detect / Genotyping PCRSpecimen Type
VariesSpecimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient | 21 days |
Reference Values
Negative for human papillomavirus (HPV) genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68
Day(s) Performed
Monday through Saturday
Report Available
3 to 6 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87626
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| AHPV | HPV Anal Detect / Genotyping PCR | 77376-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| AHPVS | Specimen Source | 31208-2 |
| 607831 | HPV High Risk type 16, PCR | 77396-0 |
| 607832 | HPV High Risk type 18, PCR | 77395-2 |
| 607833 | HPV other High Risk types, PCR | 77394-5 |