Test ID AMLMF Acute Myeloid Leukemia (AML), Specified FISH, Varies
Ordering Guidance
This test is intended for instances when limited acute myeloid leukemia (AML) fluorescence in situ hybridization (FISH) probes are needed based on specific abnormalities or on abnormalities identified in the diagnostic sample. The FISH probes to be analyzed must be specified on the request, otherwise test processing may be delayed in order to determine the intended analysis. If specific probes are not included with this test order, the test may be canceled and automatically reordered by the laboratory as either AMLAF / Acute Myeloid Leukemia (AML), FISH, Adult, Varies or AMLPF / Acute Myeloid Leukemia (AML), FISH, Pediatric, Varies depending on the age of the patient.
If the entire AML FISH panel is preferred for an adult patient, order AMLAF / Acute Myeloid Leukemia (AML), FISH, Adult, Varies.
If the entire AML FISH panel is preferred for a pediatric patient, order AMLPF / Acute Myeloid Leukemia (AML), FISH, Pediatric, Varies.
At diagnosis, conventional cytogenetic studies (CHRBM / Chromosome Analysis, Hematologic Disorders, Bone Marrow) and a complete AML FISH panel (either AMLAF or AMLPF) should be performed.
If this test is ordered and the laboratory is informed that the patient is on a Children's Oncology Group (COG) protocol, this test will be canceled and automatically reordered by the laboratory as COGMF / Acute Myeloid Leukemia (AML), Children's Oncology Group Enrollment Testing, FISH, Varies.
For testing paraffin embedded tissue samples from patients with myeloid sarcoma, order MSTF / Myeloid Sarcoma, FISH, Tissue.
Shipping Instructions
Advise Express Mail or equivalent if not on courier service.
Necessary Information
1. A list of probes requested for analysis is required. Probes available for this test are listed in the Testing Algorithm section.
2. A reason for testing and a flow cytometry and/or a bone marrow pathology report, if available, should be submitted with each specimen.The laboratory will not reject testing if this information is not provided, however appropriate testing and/or interpretation may be compromised or delayed in some instances. If this information is not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Bone marrow
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (heparin) or lavender top (EDTA)
Specimen Volume: 2 to 3 mL
Collection Instructions:
1. It is preferable to send the first aspirate from the bone marrow collection.
2. Invert several times to mix bone marrow.
Acceptable
Specimen Type: Blood
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (heparin) or lavender top (EDTA)
Specimen Volume: 6 mL
Collection Instructions: Invert several times to mix blood.
Useful For
Detecting a neoplastic clone associated with the recurrent chromosome abnormalities seen in patients with acute myeloid leukemia (AML) or other myeloid malignancies using a client specified probe set
An adjunct to conventional chromosome studies in patients with AML
Evaluating specimens in which standard cytogenetic analysis is unsuccessful
Testing Algorithm
This test includes a charge for the probe application, analysis and professional interpretation of results for 1 probe set (2 individual fluorescence in situ hybridization [FISH] probes). Additional charges will be incurred for all reflex or additional probe sets performed.
If the patient has known chromosome abnormalities identified in the diagnostic study, indicate the abnormality and which probes should be used.
When specified, any of the following probes will be performed:
t(8;21), [M2], RUNX1T1/RUNX1
Reflex: t(3;21)(q26.2;q22) MECOM/RUNX1
t(15;17), [M3], PML/RARA
Reflex: 17q21 rearrangement, RARA break-apart
11q23 rearrangement, [M0-M7], MLL (KMT2A)
Reflex: t(4;11)(q21;q23), AFF1/MLL
Reflex: t(6;11)(q27;q23), MLLT4(AFDN)/MLL
Reflex: t(9;11)(p22;q23), MLLT3/MLL
Reflex: t(10;11)(p13;q23), MLLT10/MLL
Reflex: t(11;16)(q23;p13.3), MLL/CREBBP
Reflex: t(11;19)(q23;p13.1), MLL/ELL
Reflex: t(11;19)(q23;p13.3), MLL/MLLT1
inv(16), [M4, Eos], MYH11/CBFB
Reflex: 16q22 rearrangement, CBFB break-apart
inv(16), GLIS2/CBFA2T3
11p15.4 rearrangement, NUP98 break-apart
Reflex: t(7;11)(p15;p15.4), HOXA9/NUP98
12p13 rearrangement, ETV6 break-apart
Reflex: t(7;12)(q36;p13), MNX1/ETV6
t(6;9), [M2,M4], DEK/NUP214
inv(3) or t(3;3), [M1,2,4,6,7], RPN1/MECOM
Reflex: t(1;3)(p36;q21), PRDM16/RPN1
Reflex: t(3;21)(q26.2;q22), MECOM/RUNX1
t(8;16), [M4,M5], KAT6A/CREBBP
t(1;22), [M7], RBM15/MKL1
-5/5q-, D5S630/EGR1
-7/7q-, D7Z1/ D7S486
17p-, TP53/D17Z1
t(9;22), BCR/ABL1
Reflex: 9q34 rearrangement, ABL1 break-apart
t(3;5), [M2, 4, 6], MLF1/NPM1
The following algorithms are available:
Acute Promyelocytic Leukemia: Guideline to Diagnosis and Follow-up
Special Instructions
Method Name
Fluorescence In Situ Hybridization (FISH)
Reporting Name
AML, Specified FISHSpecimen Type
VariesSpecimen Minimum Volume
Blood: 2 mL
Bone Marrow: 1 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Varies | Ambient (preferred) | ||
Refrigerated |
Reference Values
Day(s) Performed
Monday through Friday
Report Available
7 to 10 daysPerforming Laboratory

Test Classification
This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88271x2, 88275x1, 88291x1- FISH Probe, Analysis, Interpretation; 1 probe set
88271x2, 88275x1-FISH Probe, Analysis; each additional probe set (if appropriate)
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
AMLMF | AML, Specified FISH | In Process |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
614204 | Result Summary | 50397-9 |
614205 | Interpretation | 69965-2 |
614206 | Result Table | 93356-4 |
614207 | Result | 62356-1 |
GC097 | Reason for Referral | 42349-1 |
GC098 | Probes Requested | 78040-3 |
GC099 | Specimen | 31208-2 |
614208 | Source | 31208-2 |
614209 | Method | 85069-3 |
614210 | Additional Information | 48767-8 |
614211 | Disclaimer | 62364-5 |
614212 | Released By | 18771-6 |
Reflex Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
AMLMB | Probe, Each Additional (AMLMF) | No, (Bill Only) | No |
Forms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.