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Test ID ANPAT Anatomic Pathology Consultation, Wet Tissue


Ordering Guidance


For a pathology consultation on paraffin embedded tissue and slides only (ie, no wet tissue being sent to be processed), order PATHC / Pathology Consultation.



Additional Testing Requirements


 



Shipping Instructions


Vitreous fluid/aqueous humor specimens: ship overnight; Attention: Ophthalmic Pathology Working Group. Call 800-533-1710 to request an Expedite on this order since vitreous fluid will have no fixative and needs to reach the laboratory as quickly as possible.

 

Attach the green pathology address label to the outside of the transport container.



Necessary Information


The following information is required:

 

1. All requisitions must be labeled with:

-Patient name, date of birth and medical record number

-Name and phone number of the referring pathologist or ordering physician

-Anatomic site and collection date

 

2. All specimens must be labeled with:

-Two patient identifiers (first and last name, date of birth or medical record number)

-Specimen type and anatomic site

 

3. A brief patient history is essential to achieve a consultation fully relevant to the ordering physician's needs.

4. A preliminary pathology report is preferred, if available, in order to ensure that the specimen is known to a referring pathologist.

 

All requisition and supporting information must be submitted in English.



Specimen Required


Specimen Type: Lung biopsy

Supplies:

-Pathology Packaging Kit (T554)

-Formalin, 10% (T118)

-Michel's Transport Media for Immunofluorescent Testing on Tissue (T321)

Specimen Volume: Entire specimen

Collection Instructions:

1. Submit portion of lung tissue in 10% neutral buffered formalin for light microscopy processing. Alternatively, submit a representative hematoxylin and eosin (H and E) slide from the light microscopy sample along with the tissue in Michel's transport media. Pathology Packaging Kit can be used to submit H and E slide.

2. Place a portion of the unfixed lung tissue in Michel's transport media for immunofluorescence.

 

Specimen Type: Cardiac biopsy

Supplies:

-Pathology Packaging Kit (T554)

-Formalin, 10% (T118)

-Michel's Transport Media for Immunofluorescent Testing on Tissue (T321)

-Electron Microscopy Kit (T660)

-Gluta (Trumps) (T130)

Specimen Volume: Entire specimen

Collection Instructions:

1. For ideal analysis, collect 4 to 6 biopsy specimens and submit all in 10% neutral buffered formalin unless electron microscopy (EM) is requested.

2. If EM is needed, submit at least 1 biopsy into 2.5% to 3% buffered glutaraldehyde preservative using the Electron Microscopy Kit along with the formalin biopsy specimens. If formalin biopsy specimens are not available, light microscopic slides would also be acceptable.

Additional Information: If the question is drug toxicity, storage disease, or if the patient is younger than 25 years old, send at least 1 biopsy specimen in 2.5% to 3% buffered glutaraldehyde in case EM is needed for diagnosis.

 

Specimen Type: Cardiac explant

Supplies:

-Pathology Packaging Kit (T554)

-Refrigerate/Ambient Shipping Box, 5 lb (T329)

Specimen Volume: Entire specimen

Collection Instructions:

1. Fix entire explant specimen in formalin for a minimum of 24 hours.

2. Following fixation, lightly wrap specimen in formalin-soaked paper towel, place in a leak-proof sealable bag with a small amount of formalin, seal, and ship ambient in a sturdy shipping container (ie, Refrigerate/Ambient Shipping Box, 5 lb).

Additional Information: Include the last pretransplantation echocardiogram with report and images as well as all other relevant clinical documents.

 

Specimen Type: Enucleated eye

Supplies:

-Pathology Packaging Kit (T554)

-Formalin - 10% Histo Prep, 45 mL (T117)

Specimen Volume: Entire specimen

Collection Instructions:

1. Immediately after removal, place specimen in approximately 300 mL of 10% neutral buffered formalin.

2. Enucleated eye should not be opened or punctured.

3. After 48 hours in the originating laboratory, the specimen should be transferred from the larger, 300 mL container to a smaller container with fixative (Formalin - 10% Histo Prep, 45 mL) to be shipped without risk of leakage.

 

Specimen Type: Iris, conjunctiva, cornea, and other small biopsies from eye

Supplies:

-Pathology Packaging Kit (T554)

-Formalin - 10% Histo Prep, 45 mL (T117)

Specimen Volume: Entire specimen

Collection Instructions:

1. Obtain biopsy.

2. Place iris, conjunctiva, cornea, and other small biopsies on a paper mount (a piece of filter paper or other porous paper). This helps to keep the specimen from curling when it is fixed.

3. Immediately but gently place specimen (on the paper mount) into 10% formalin, approximately 20 times the volume of the biopsy (Formalin - 10% Histo Prep, 45 mL).

4. In cases where margins are important, placing sutures at the margins of resection with accompanying explanatory draft will help orientation of the specimen in the lab.

5. Patient history and clinical/surgical notes should be included if available.

 

Specimen Type: Vitreous fluid/Aqueous humor

Supplies:

-Pathology Packaging Kit (T554)

-Sarstedt Aliquot Tube, 5 mL (T914)

Specimen Volume: Entire specimen

Collection Instructions:

1. Obtain liquid specimen.

2. Place the liquid (vitreous or aqueous) concentrate into a small tube with a screw-top cap. Seal the tube tightly.

3. Put the specimen immediately on a refrigerated cool pack (not dry ice or frozen cool pack).

4. If a washing (diluted) is available, it can also be sent in similar manner.

Specimen Stability Information: Refrigerated


Forms

For heart specimens, if not ordering electronically, complete, print, and send a Cardiovascular Test Request (T724) with the specimen.

Useful For

Obtaining a rapid, expert opinion on unprocessed specimens (lung tissue for immunofluorescence, cardiac biopsies, enucleated eye specimens, iris, conjunctiva, cornea, and other small eye biopsies) referred by a pathologist

 

This test is not useful for suspected hematologic disorders.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
LEV1P Level 1 Gross only No, (Bill Only) No
LEV2P Level 2 Gross and microscopic No, (Bill Only) No
LEV3P Level 3 Gross and microscopic No, (Bill Only) No
LEV4P Level 4 Gross and microscopic No, (Bill Only) No
LEV5P Level 5 Gross and microscopic No, (Bill Only) No
LEV6P Level 6 Gross and microscopic No, (Bill Only) No

Testing Algorithm

Laboratory approval is required prior to ordering this test. Contact Mayo Clinic Laboratories at 800-533-1710.

An interpretation will be provided by Mayo Clinic staff pathologists within a formal pathology report sent to the referring pathologist.

 

Appropriate stains and other ancillary testing may be performed at an additional charge.

 

Note: Tests requested by the referring physician (immunostains, molecular studies, etc.) may not be performed if they are deemed to be unnecessary by the reviewing Mayo Clinic pathologist. For all pathology consultations, ancillary testing is ordered at the discretion of the Mayo Clinic pathologist.

 

Note: Calls are not routinely made; however, depending on the nature of the case, a call may be placed to the ordering provider or pathologist. To contact a Mayo Clinic Pathologist, contact Mayo Clinic Laboratories at 800-533-1710.

 

For more information see Pathology Consultation Ordering Algorithm.

Reporting Name

Wet Tissue Pathology Consult

Specimen Type

Varies

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient (preferred)
  Frozen 
  Refrigerated 

Report Available

3 days; Cases requiring additional material or ancillary testing may require additional time.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

88300 (if appropriate)

88302 (if appropriate)

88304 (if appropriate)

88305 (if appropriate)

88307 (if appropriate)

88309 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ANPAT Wet Tissue Pathology Consult In Process

 

Result ID Test Result Name Result LOINC Value
71043 Interpretation 60570-9
71044 Participated in the Interpretation No LOINC Needed
71045 Report Electronically signed by 19139-5
71046 Addendum 35265-8
71047 Gross Description 22634-0
71048 Material Received 22633-2
71584 Disclaimer 62364-5
71790 Case Number 80398-1

Reference Values

The laboratory will provide a pathology consultation.

Day(s) Performed

Monday through Friday

Test Classification

Not Applicable

Method Name

Medical Interpretation