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Test ID ATPCO Anal ThinPrep Cytology with Human Papillomavirus (HPV) Co-Test, Varies

Necessary Information

1. The following information must accompany the specimen: Patient's name, medical record number, date of birth, sex, source (exact location and procedure used), date specimen was taken, name of ordering physician and pager number.

2. Submit any pertinent history or clinical information.

Specimen Required

Supplies: PreservCyt Vial (T536)

Specimen Type: Anus or rectum

Container/Tube: ThinPrep/PreservCyt solution vial

Specimen Volume: A minimum of 20 mL or entire collection

Collection Instructions:

1. Visualize the anal opening by retracting the buttocks.

2. Moisten Dacron or polyester swab past internal anal sphincter until it abuts the distal rectal wall (4-5cm).

3. Rotate the swab 360 degrees and maintain lateral pressure on swab against the walls of the anus. The swab should bow slightly due to the pressure.

4. While rotating, slowly withdraw the swab.

5. Rotate at least 10 times while withdrawing the swab. This should take 20 to 30 seconds.

6. Immediately place swab in the ThinPrep solution. Swish at least 20 to 30 seconds in the ThinPrep solution.

7. Dispose of the swab.

8. Tighten the cap so that the torque line on the cap passes the torque line on the vial.

9. Place labels on the vial and on the bag.

10. Bag ThinPrep specimens individually as they tend to leak during transport.

Useful For

Detection of malignant and premalignant changes


Detection of high-risk (HR) genotypes associated with the development of anal cancer


Individual genotyping of human papillomavirus (HPV)-16 and HPV-18, if present


May aid in triaging men and women with positive HR-HPV but negative anal Pap smear results


The cobas HPV test is not recommended for evaluation of suspected sexual abuse.

Additional Tests

Test ID Reporting Name Available Separately Always Performed
AHPV HPV Anal Detect / Genotyping PCR No Yes

Testing Algorithm

When this test is ordered, a cytology screen and genotyping for high-risk human papillomavirus types will be performed.

Method Name

ATPCO: Light Microscopy

AHPV: Real-Time Polymerase Chain Reaction (PCR)

Reporting Name

Anal ThinPrep Cytology w/HPV CoTest

Specimen Type


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient 21 days THIN PREP

Reference Values


Satisfactory for evaluation. Negative for intraepithelial lesion or malignancy.



Negative for HPV genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68

Day(s) Performed

Monday through Friday

Report Available

3 to 6 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

ATPCO – 88112

AHPV - 87624

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ATPCO Anal ThinPrep Cytology w/HPV CoTest In Process


Result ID Test Result Name Result LOINC Value
614403 Interpretation 69965-2
614404 Participated in the Interpretation No LOINC Needed
614405 Report electronically signed by 19139-5
614406 Addendum 35265-8
614407 Gross Description 22634-0
CY088 Source 22633-2
CY089 Clinical History 22636-5
614408 Disclaimer 62364-5
614409 Case Number 80398-1