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Test ID BAPS Bile Acid Profile, Serum

Ordering Guidance

This test is intended for use by research scientists. Approval must be obtained before ordering.

Specimen Required

Patient Preparation: Patient must be fasting for 12 to 14 hours.

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.

Useful For

Evaluating the enterohepatic cycle consisting of the biliary system, intestine, portal circulation, and hepatocytes


Supporting researchers in need of free and conjugated values of all 20 bile acid species as well as total bile acid

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Bile Acid Profile, S

Specimen Type


Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 90 days
  Ambient  90 days
  Frozen  90 days

Reference Values

Chenodeoxycholic acid: ≤2.26 nmol/mL

Cholic acid: ≤2.74 nmol/mL

Deoxycholic acid: ≤2.84 nmol/mL

Dihydroxycholestanoic acid: ≤0.07 nmol/mL

Glycochenodeoxycholic acid: ≤5.14 nmol/mL

Glycocholic acid: ≤2.17 nmol/mL

Glycodeoxycholic acid: ≤3.88 nmol/mL

Glycohyodeoxycholic acid: ≤0.01 nmol/mL

Glycolithocholic acid: ≤0.11 nmol/mL

Glycoursodeoxycholic acid: ≤1.00 nmol/mL

Hyodeoxycholic acid: ≤0.12 nmol/mL

Lithocholic acid: ≤0.09 nmol/mL

Taurochenodeoxycholic acid: ≤0.80 nmol/mL

Taurocholic acid: ≤0.31 nmol/mL

Taurodeoxycholic acid: ≤0.78 nmol/mL

Taurohyodeoxycholic acid: ≤0.02 nmol/mL

Taurolithocholic acid: ≤0.04 nmol/mL

Tauroursodeoxycholic acid: ≤0.05 nmol/mL

Trihydroxycholestanoic acid: ≤1.73 nmol/mL

Ursodeoxycholic acid: ≤0.64 nmol/mL

Total bile acids: ≤19.00 nmol/mL

Day(s) Performed

Monday through Friday

Report Available

3 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
BAPS Bile Acid Profile, S 43130-4


Result ID Test Result Name Result LOINC Value
35802 Chenodeoxycholic acid 30519-3
35801 Cholic acid 30518-5
35803 Deoxycholic acid 30520-1
35819 Dihydroxycholestanoic acid 53479-2
35808 Glycochenodeoxycholic acid 93335-8
35807 Glycocholic acid 93334-1
35809 Glycodeoxycholic acid 93333-3
35811 Glycohyodeoxycholic acid 93332-5
35812 Glycolithocholic acid 93331-7
35810 Glycoursodeoxycholic acid 93330-9
35805 Hyodeoxycholic acid 93329-1
35806 Lithocholic acid 74897-0
35814 Taurochenodeoxycholic acid 93328-3
35813 Taurocholic acid 93327-5
35815 Taurodeoxycholic acid 93326-7
35817 Taurohyodeoxycholic acid 93325-9
35818 Taurolithocholic acid 93324-2
35816 Tauroursodeoxycholic acid 93323-4
35820 Trihydroxycholestanoic acid 38188-9
35804 Ursodeoxycholic acid 55159-8
35821 Total bile acids 14628-2