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Test ID BIOTN Biotin, Serum

Ordering Guidance

If testing for biotinidase deficiency is requested, order BIOTS / Biotinidase, Serum.

Shipping Instructions

Freeze specimens within 3 days of collection and send frozen.

Specimen Required

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.

Useful For

Measurement of biotin in serum


Assessment of biotin concentrations in individuals taking biotin supplements


Investigation of unexpected results from immunoassays that utilize biotin-streptavidin detection methods


This test is not useful as a screen for biotinidase deficiency.

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Stable Isotope Dilution Analysis

Reporting Name

Biotin, S

Specimen Type


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Frozen (preferred) 30 days
  Refrigerated  7 days
  Ambient  24 hours

Reference Values

≥18 years: ≤0.3 ng/mL

Reference values have not been established for patients who are <18 years of age.

Day(s) Performed


Report Available

2 to 7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
BIOTN Biotin, S 1980-2


Result ID Test Result Name Result LOINC Value
606867 Biotin, S 1980-2