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Test ID BNZU Benzodiazepines Confirmation, Random, Urine

Ordering Guidance

For situations where chain of custody is required, a Chain of Custody Kit (T282) is available. For chain-of-custody testing, order BNZX / Benzodiazepines Confirmation, Chain of Custody, Random, Urine.


Additional drug panels and specific requests are available. Call 800-533-1710 or 507-266-5700.

Additional Testing Requirements

If urine creatinine is required or adulteration of the sample is suspected, order ADULT / Adulterants Survey, Random, Urine.

Specimen Required

Supplies: Aliquot Tube, 5 mL (T465)

Collection Container/Tube: Plastic urine container

Submission Container/Tube: Plastic, 5-mL tube

Specimen Volume: 1 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative

Additional Information:

1. No specimen substitutions.

2. STAT requests are not accepted for this test.

3. Submitting less than 1 mL will compromise our ability to perform all necessary testing.

Useful For

Detecting drug use involving benzodiazepines such as alprazolam, chlordiazepoxide, clonazepam, diazepam, midazolam, oxazepam, temazepam, clobazam, flunitrazepam, flurazepam, lorazepam, prazepam, triazolam, and zolpidem

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Benzodiazepines Confirmation, U

Specimen Type


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 7 days
  Frozen  14 days

Reference Values



Cutoff concentrations by liquid chromatography- tandem mass spectroscopy:

Alprazolam: 10 ng/mL

Alpha-hydroxyalprazolam: 10 ng/mL

Chlordiazepoxide: 10 ng/mL

Clonazepam: 10 ng/mL

7-Aminoclonazepam: 10 ng/mL

Diazepam: 10 ng/mL

Nordiazepam: 10 ng/mL

Midazolam: 10 ng/mL

Alpha-hydroxy midazolam: 10 ng/mL

Oxazepam: 10 ng/mL

Temazepam: 10 ng/mL

Clobazam: 10 ng/mL

N-Desmethylclobazam by LC-MS/MS: 10 ng/mL

Flunitrazepam: 10 ng/mL

7-Aminoflunitrazepam: 10 ng/mL

Flurazepam: 10 ng/mL

2-Hydroxy ethyl flurazepam: 10 ng/mL

Lorazepam: 10 ng/mL

Prazepam: 10 ng/mL

Triazolam: 10 ng/mL

Alpha-hydroxy triazolam: 10 ng/mL

Zolpidem: 10 ng/mL

Zolpidem phenyl-4-carboxylic acid: 10 ng/mL

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information




G0480 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BNZU Benzodiazepines Confirmation, U 90890-5


Result ID Test Result Name Result LOINC Value
608256 Alprazolam by LC-MS/MS 59615-5
608257 Alpha-Hydroxyalprazolam by LC-MS/MS 16348-5
608258 Chlordiazepoxide by LC-MS/MS 20522-9
608259 Clonazepam by LC-MS/MS 16229-7
608260 7-aminoclonazepam by LC-MS/MS 51776-3
608261 Diazepam by LC-MS/MS 16227-1
608262 Nordiazepam by LC-MS/MS 16228-9
608263 Midazolam by LC-MS/MS 16233-9
608264 Alpha-Hydroxy Midazolam by LC-MS/MS 59590-0
608265 Oxazepam by LC-MS/MS 16201-6
608266 Temazepam by LC-MS/MS 20559-1
608267 Clobazam by LC-MS/MS 59635-3
608268 N-Desmethylclobazam by LC-MS/MS 97160-6
608269 Flunitrazepam by LC-MS/MS 20528-6
608270 7-aminoflunitrazepam by LC-MS/MS 51777-1
608271 Flurazepam by LC-MS/MS 16231-3
608272 2-Hydroxy Ethyl Flurazepam by LC-MS/MS 97159-8
608273 Lorazepam by LC-MS/MS 17088-6
608274 Prazepam by LC-MS/MS 17479-7
608275 Triazolam by LC-MS/MS 16232-1
608276 Alpha-Hydroxy Triazolam by LC-MS/MS 49876-6
608277 Zolpidem by LC-MS/MS 72770-1
608278 Zolpidem Phenyl-4-Carboxylic acid by LC-MS/MS 72768-5
608448 Benzodiazepines Interpretation 16195-0

Day(s) Performed

Tuesday through Friday, Sunday

Report Available

3 to 6 days