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Test ID BNZX Benzodiazepines Confirmation, Chain of Custody, Random, Urine

Specimen Required

Supplies: Chain of Custody Kit (T282)

Container/Tube: Chain of custody kit containing the specimen containers, seals, and documentation required

Specimen Volume: 5 mL

Collection Instructions: Collect specimen in the container provided, seal, and submit with the associated documentation to satisfy the legal requirements for chain-of-custody testing.

Additional Information: Submitting less than 5 mL will compromise the ability to perform all necessary testing.


1. Chain of Custody Request is included in the Chain of Custody Kit (T282).

2. If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.

Useful For

Detecting drug use involving benzodiazepines such as alprazolam, chlordiazepoxide, clonazepam, diazepam, midazolam, oxazepam, temazepam, clobazam, flunitrazepam, flurazepam, lorazepam, prazepam, triazolam, and zolpidem, in urine specimens handled through the chain-of-custody process


Providing chain of custody for when the results of testing could be used in a court of law. Its purpose is to protect the rights of the individual contributing the specimen by demonstrating that it was under the control of personnel involved with testing the specimen at all times; this control implies that the opportunity for specimen tampering would be limited.

Additional Tests

Test ID Reporting Name Available Separately Always Performed
COCH Chain of Custody Processing No Yes
ADLTX Adulterants Survey, CoC, U Yes Yes

Testing Algorithm

Testing for adulterants will be performed on all chain-of-custody urine samples per regulatory requirements.

Method Name

BNZX: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

ADLTX: Spectrophotometry

Reporting Name

Benzodiazepines Conf, CoC, U

Specimen Type


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 7 days
  Frozen  14 days

Reference Values



Cutoff concentrations by liquid chromatography- tandem mass spectroscopy:

Alprazolam: 10 ng/mL

Alpha-hydroxyalprazolam: 10 ng/mL

Chlordiazepoxide: 10 ng/mL

Clonazepam: 10 ng/mL

7-Aminoclonazepam: 10 ng/mL

Diazepam: 10 ng/mL

Nordiazepam: 10 ng/mL

Midazolam: 10 ng/mL

Alpha-hydroxy midazolam: 10 ng/mL

Oxazepam: 10 ng/mL

Temazepam: 10 ng/mL

Clobazam: 10 ng/mL

N-Desmethylclobazam by LC-MS/MS: 10 ng/mL

Flunitrazepam: 10 ng/mL

7-Aminoflunitrazepam: 10 ng/mL

Flurazepam: 10 ng/mL

2-Hydroxy ethyl flurazepam: 10 ng/mL

Lorazepam: 10 ng/mL

Prazepam: 10 ng/mL

Triazolam: 10 ng/mL

Alpha-hydroxy triazolam: 10 ng/mL

Zolpidem: 10 ng/mL

Zolpidem phenyl-4-carboxylic acid: 10 ng/mL

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


G0480 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BNZX Benzodiazepines Conf, CoC, U 90890-5


Result ID Test Result Name Result LOINC Value
608452 Benzodiazepines Immunoassay Screen 14316-4
608280 Alprazolam by LC-MS/MS 59615-5
608281 Alpha-Hydroxyalprazolam by LC-MS/MS 16348-5
608282 Chlordiazepoxide by LC-MS/MS 20522-9
608283 Clonazepam by LC-MS/MS 16229-7
608284 7-aminoclonazepam by LC-MS/MS 51776-3
608285 Diazepam by LC-MS/MS 16227-1
608286 Nordiazepam by LC-MS/MS 16228-9
608287 Midazolam by LC-MS/MS 16233-9
608288 Alpha-Hydroxy Midazolam by LC-MS/MS 59590-0
608289 Oxazepam by LC-MS/MS 16201-6
608290 Temazepam by LC-MS/MS 20559-1
608291 Clobazam by LC-MS/MS 59635-3
608292 N-Desmethylclobazam by LC-MS/MS 97160-6
608293 Flunitrazepam by LC-MS/MS 20528-6
608294 7-aminoflunitrazepam by LC-MS/MS 51777-1
608295 Flurazepam by LC-MS/MS 16231-3
608296 2-Hydroxy Ethyl Flurazepam by LC-MS/MS 97159-8
608297 Lorazepam by LC-MS/MS 17088-6
608298 Prazepam by LC-MS/MS 17479-7
608299 Triazolam by LC-MS/MS 16232-1
608300 Alpha-Hydroxy Triazolam by LC-MS/MS 49876-6
608301 Zolpidem by LC-MS/MS 72770-1
608302 Zolpidem Phenyl-4-Carboxylic acid by LC-MS/MS 72768-5
608449 Benzodiazepines Interpretation 16195-0
608450 Chain of Custody 77202-0

Day(s) Performed

Tuesday through Friday, Sunday

Report Available

3 to 6 days