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Test ID BPRPV Bordetella pertussis and Bordetella parapertussis, Molecular Detection, PCR, Varies

Necessary Information

Specimen source is required.

Specimen Required

The high sensitivity of amplification by polymerase chain reaction (PCR) requires the specimen to be processed in an environment in which contamination of the specimen by Bordetella pertussis or Bordetella parapertussis DNA is unlikely.


Submit only 1 of the following specimens:



Specimen Type: Nasopharyngeal swab


Culture Swab - Liquid Stuarts/Single Swab (NP Swab) (T515)

Container/Tube: Rayon swab with an aluminum shaft placed in transport medium such as a green-top nasopharyngeal swab (rayon mini-tip) with Stuart's media (no charcoal), or Stuart's media with charcoal, or Amies media with or without charcoal (Transwab Nasopharyngeal with Charcoal System).

Additional Information:

1. Swab transport containers without charcoal must contain a pledget saturated with either Stuart's or Amies liquid media. Clear semi-solid/solid media is gel and will be rejected.

2. Other swab or media types may be inhibitory to PCR testing and will be rejected.



Specimen Type: Nasopharyngeal (not throat) aspirate/wash or nasal aspirate/wash

Container/Tube: Sterile container with a screw top cap (no transport media)

Specimen Volume: Entire collection

Useful For

Preferred diagnostic test for the detection of Bordetella pertussis or Bordetella parapertussis


This test is not recommended for screening asymptomatic individuals who may carry B pertussis or parapertussis.


This test is not recommended for follow up of patients previously diagnosed with pertussis (ie, as a test of cure).

Method Name

Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Reporting Name

Bordetella, PCR, Varies

Specimen Type


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Ambient  7 days
  Frozen  7 days

Reference Values

Not applicable

Day(s) Performed

Monday through Friday, Sunday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87798 x 2

87999 (if appropriate for government payers)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BPRPV Bordetella, PCR, Varies 90441-7


Result ID Test Result Name Result LOINC Value
BPRS Specimen source 31208-2
618312 Bordetella pertussis PCR 43913-3
618313 Bordetella parapertussis PCR 42588-4


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.