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Test ID CIFA Cutaneous Immunofluorescence Antibodies, IgA, Serum

Specimen Required

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.

Useful For

Confirming the presence of IgA antibodies to diagnose pemphigoid, pemphigus, epidermolysis bullosa acquisita, or bullous lupus erythematosus

Method Name

Indirect Immunofluorescence Assay (IFA)

Reporting Name

Cutaneous Immfluor. Ab (IgA), S

Specimen Type


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  30 days
  Ambient  14 days

Reference Values

Report includes presence and titer of circulating antibodies. If serum contains basement membrane zone (BMZ) antibodies on split-skin substrate, patterns will be reported as:

1) Epidermal pattern, consistent with pemphigoid

2) Dermal pattern, consistent with epidermolysis bullosa acquisita


Negative in normal individuals

Day(s) Performed

Monday through Friday

Report Available

2 to 7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information



LOINC Code Information

Test ID Test Order Name Order LOINC Value
CIFA Cutaneous Immfluor. Ab (IgA), S In Process


Result ID Test Result Name Result LOINC Value
610628 Cell Surface Ab IgA In Process
610629 Basement Membrane IgA In Process
610630 Primate Esophagus IgA In Process
610631 Primate Split Skin IgA In Process
610632 Other 48767-8