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HelpTest ID CLLDF Chronic Lymphocytic Leukemia, Diagnostic FISH, Varies
Ordering Guidance
If testing a paraffin-embedded tissue specimen for patients with chronic lymphocytic leukemia (CLL) is desired, order SLL / Small Lymphocytic Lymphoma, FISH, Tissue.
Shipping Instructions
Advise Express Mail or equivalent if not on courier service.
Necessary Information
1. Provide a reason for testing with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
2. A pathology and/or flow cytometry report may be requested by the Genomics Laboratory to optimize testing and aid in interpretation of results.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Blood
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (sodium heparin), purple top (EDTA)
Specimen Volume: 6 mL
Collection Instructions: Invert several times to mix blood.
Specimen Type: Bone marrow
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (sodium heparin), purple top (EDTA)
Specimen Volume: 1-2 mL
Collection Instructions: Invert several times to mix bone marrow.
Useful For
Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with chronic lymphocytic leukemia (CLL)
Identifying and tracking known chromosome abnormalities in patients with CLL and tracking response to therapy
Distinguishing patients with 11;14 translocations who have leukemic phase of mantle cell lymphoma from patients who have CLL
Detecting patients with atypical CLL or other forms of lymphoma associated with translocations between IGH and BCL3
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CLLDB | Probe, Each Additional (CLLDF) | No, (Bill Only) | No |
Testing Algorithm
This test includes a charge for the probe application, analysis, and professional interpretation of results for 6 probe sets (12 individual fluorescence in situ hybridization probes). Additional charges will be incurred for all reflex or additional probe sets performed.
Panel includes testing for the following abnormalities using the probes listed:
6q-, D6Z1/MYB
11q-, D11Z1/ATM
+12, D12Z3/MDM2
13q-, D13S319/LAMP1
17p-, TP53/D17Z1
t(11;14), CCND1/IGH
When an additional IGH signal is identified in the absence of a CCND1/IGH fusion, reflex testing using the IGH/BCL3 probe set will be performed to identify a potential IGH/BCL3 fusion [t(14;19)(q32;q13)].
This assay detects abnormalities observed in the blood and bone marrow of patients with chronic lymphocytic leukemia. If a paraffin-embedded tissue sample is received, this test will be cancelled and SLL / Small Lymphocytic Lymphoma, FISH, Tissue will be added and performed as the appropriate test.
Method Name
Reporting Name
CLL, Diagnostic FISHSpecimen Type
VariesSpecimen Minimum Volume
Blood: 2 mL
Bone Marrow: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Friday
Report Available
7 to 10 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88271 x 12
88275 x 6
88291 - FISH Probe, Analysis, Interpretation; 6 probe sets
88271 x 2 (if appropriate)
88275 x1 - FISH Probe, Analysis; each additional probe set (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CLLDF | CLL, Diagnostic FISH | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 610714 | Result Summary | 50397-9 |
| 610715 | Interpretation | 69965-2 |
| 610716 | Result Table | 93356-4 |
| 610717 | Result | 62356-1 |
| GC088 | Reason for Referral | 42349-1 |
| GC089 | Specimen | 31208-2 |
| 610718 | Source | 85298-8 |
| 610719 | Method | 85069-3 |
| 610720 | Additional Information | 48767-8 |
| 610721 | Disclaimer | 62364-5 |
| 610722 | Released by | 18771-6 |
Forms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.