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Test ID EEEV1 Red Blood Cell (RBC) Enzyme Evaluation, Blood

Necessary Information

Metabolic Hematology Patient Information (T810) is strongly recommended and should include clinical history. Testing may proceed without this information, however if the information requested is received, it allows for a more complete interpretation.

Specimen Required


Preferred: Yellow top (ACD solution B)

Acceptable: Yellow top (ACD solution A)

Specimen Volume: 12 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


1. Metabolic Hematology Patient Information (T810)

2. If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.

Useful For

Identifying defects of red blood cell enzyme metabolism


Evaluating patients with Coombs-negative hemolytic anemia

Profile Information

Test ID Reporting Name Available Separately Always Performed
EEEVI Erythrocyte Enzyme Interpretation No Yes
G6PDC G6PD Enzyme Activity, B Yes, (Order G6PD1) Yes
PKC PK Enzyme Activity, B Yes, (Order PK1) Yes
GPIC Glucose Phosphate Isomerase, B Yes, (Order GPI1) Yes
HKC Hexokinase, B Yes, (Order HK1) Yes
AKC Adenylate Kinase, B Yes, (Order AK1) Yes
PFKC Phosphofructokinase, B Yes, (Order PFK1) Yes
PGKC Phosphoglycerate Kinase, B Yes, (Order PGK1) Yes
TPIC Triosephosphate Isomerase, B Yes, (Order TPI1) Yes
GSH Glutathione, B Yes Yes
P5NT Pyrimidine 5' Nucleotidase, B Yes Yes

Testing Algorithm

This is a consultative evaluation in which the case will be evaluated at Mayo Clinic Laboratories.

Method Name

EEEVI: Medical Interpretation

G6PDC, GPIC, PKC, HKC, AKC, PFKC, PGKC, TPIC, GSH, P5NT: Kinetic Spectrophotometry

Reporting Name

RBC Enzyme Evaluation

Specimen Type

Whole Blood ACD-B

Specimen Minimum Volume

5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood ACD-B Refrigerated 11 days

Reference Values

Definitive results and an interpretive report will be provided.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

82955-G6PD Enzyme Activity

84087-Glucose phosphate isomerase

84220-Pyruvate Kinase Enzyme Activity


82657-Adenylate Kinase


82657-Phosphoglycerate Kinase

82657-Triosephosphate Isomerase


83915-Pyrimidine 5' Nucleotidase

LOINC Code Information

Test ID Test Order Name Order LOINC Value
EEEV1 RBC Enzyme Evaluation 72695-0


Result ID Test Result Name Result LOINC Value
PKCL PK Enzyme Activity, B 32552-2
GPICL Glucose Phosphate Isomerase, B 44050-3
G6PCL G6PD Enzyme Activity, B 32546-4
608409 Glutathione, B 2383-8
TPICL Triosephosphate Isomerase, B 44054-5
PGKCL Phosphoglycerate Kinase, B 44053-7
PFKCL Phosphofructokinase, B 72664-6
HKCL Hexokinase, B 49216-5
2734 Pyrimidine 5' Nucleotidase, B 2902-5
AKCL Adenylate Kinase, B 44051-1
608087 Erythrocyte Enzyme Interpretation 59466-3
608109 Reviewed By 18771-6

Day(s) Performed

Tuesday, Thursday

Report Available

2 to 13 days