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Test ID EOSMF Chronic Eosinophilia, Specified FISH, Varies

Ordering Guidance

This test is intended for instances when limited chronic eosinophilia fluorescence in situ hybridization (FISH) probes are needed. The FISH probes to be analyzed must be specified on the request, otherwise test processing may be delayed in order to determine the intended analysis. If specific probes are not included with this test request, this test may be canceled and automatically reordered by the laboratory as EOSDF / Chronic Eosinophilia, Diagnostic FISH, Varies.


If the entire chronic eosinophilia FISH panel is preferred, order EOSDF / Chronic Eosinophilia, Diagnostic, FISH, Varies.


Paraffin embedded tissue testing is not available for these probe sets.


This test is ordered for targeted FISH probes to be evaluated based on specific abnormalities or on abnormalities identified in the diagnostic sample.


At diagnosis, a EOSDF / Chronic Eosinophilia, Diagnostic, FISH, Varies panel and a conventional chromosome study should be performed.

Shipping Instructions

Advise Express Mail or equivalent if not on courier service.

Necessary Information

1. A list of probes requested for analysis is required. Probes available for this test are listed in the Testing Algorithm section.

2. A reason for testing should be with each specimen. The laboratory will not reject testing if this information is not provided; however, appropriate testing and interpretation may be compromised or delayed. If not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.

3. A pathology and/or flow cytometry report may be requested by the laboratory to optimize testing and aid in interpretation of results.

Specimen Required

Submit only 1 of the following specimens:



Specimen Type: Bone marrow


Preferred: Yellow top (ACD)

Acceptable: Green top (heparin) or lavender top (EDTA)

Specimen Volume: 2-3 mL

Collection Instructions:

1. It is preferable to send the first aspirate from the bone marrow collection.

2. Invert several times to mix bone marrow.

3. Send bone marrow specimen in original tube. Do not aliquot.



Specimen Type: Blood


Preferred: Yellow top (ACD)

Acceptable: Green top (heparin) or lavender top (EDTA)

Specimen Volume: 6 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in original tube. Do not aliquot.

Useful For

Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with myeloid/lymphoid neoplasms with eosinophilia and gene rearrangement (including PDGFRA, PDGFRB, FGFR1, JAK2, and ABL1) using specified probes set


Supporting a diagnosis of malignancy when a clone is present


An adjunct to conventional chromosome studies

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
EOSMB Probe, Each Additional (EOSMF) No, (Bill Only) No
EOS3B Probe, Tri-color (EOSMF) No, (Bill Only) No

Testing Algorithm

This test includes a charge for the probe application, analysis, and professional interpretation of results for 1 probe set (2 individual fluorescence in situ hybridization [FISH] probes or 3 individual FISH probes). Additional charges will be incurred for all reflex or additional probe sets performed.


If the patient is being treated for known abnormalities, indicate the abnormality and which probes should be used.


When specified, any of the following probes will be performed:

4q12 deletion or rearrangement, FIP1L1, CHIC2, PDGFRA

4q12 rearrangement, PDGFRA

5q32 rearrangement, PDGFRB

t(5;12), PDGFRB/ETV6

8p11.2 rearrangement, FGFR1

9p24.1 rearrangement, JAK2

9q34 rearrangement, ABL1

t(9;22), BCR/ABL1


For more information see Eosinophilia: Bone Marrow Diagnostic Algorithm.

Method Name

Fluorescence In Situ Hybridization (FISH)

Reporting Name

Chronic Eosinophilia, Spec FISH

Specimen Type


Specimen Minimum Volume

Blood: 2 mL
Bone Marrow: 1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient (preferred)

Reference Values

An interpretive report will be provided.

Day(s) Performed

Monday through Friday

Report Available

7 to 10 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88271 x2, 88275 x1, 88291 x1-FISH Probe, Analysis, Interpretation; 1 probe sets

88271 x2, 88275 x1-FISH Probe, Analysis; each additional probe set (if appropriate)

88271 x1-FISH Probe; coverage for sets containing 3 probes (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
EOSMF Chronic Eosinophilia, Spec FISH In Process


Result ID Test Result Name Result LOINC Value
614256 Result Summary 50397-9
614257 Interpretation 69965-2
614258 Result Table 93356-4
614259 Result 62356-1
GC113 Reason for Referral 42349-1
GC114 Probes Requested 78040-3
GC115 Specimen 31208-2
614260 Source 31208-2
614261 Method 85069-3
614262 Additional Information 48767-8
614263 Disclaimer 62364-5
614264 Released By 18771-6


If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.