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Test ID ESBLS Extended-Spectrum Beta-Lactamase-Producing Gram-Negative Bacteria Surveillance Culture, Feces

Shipping Instructions

Specimen must arrive within 96 hours of collection.

Specimen Required

Patient Preparation: Do not use barium or bismuth before specimen collection.

Supplies: Culture and Sensitivity Stool Transport Vial (T058)

Specimen Type: Preserved feces

Container/Tube: Cary-Blair or modified Cary-Blair transport system is required.

Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of nonnutritive transport medium containing phenol red as a pH indicator. Submit sample in original Cary Blair medium container (not an aliquot of Cary Blair medium).

Specimen Volume: Representative portion of feces; 1 gram or 5 mL

Collection Instructions:

1. Collect fresh fecal specimen and place 1 gram or 5 mL in container with transport medium.

2. Place feces in preservative within 2 hours of collection.

3. Place vial in a sealed plastic bag.

Useful For

Screening for colonization of extended-spectrum beta-lactamase (ESBL)-producing organisms in stool


Screening fecal microbiota transplant donor feces for ESBL-producing organisms.


This test is not intended for medicolegal use.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No
CHESB ESBL SUS Test Charge No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed at an additional charge. All bacterial isolates tested, to rule in or out as a potential extended-spectrum beta-lactamase (ESBL)-producing pathogen, will be billed for work performed and resources consumed. The following Enterobacterales will be tested for ESBL production: Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, and Proteus mirabilis.

Method Name

Conventional Culture Technique

Reporting Name

ESBL GNB Surveillance Culture, F

Specimen Type


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Fecal Ambient (preferred) 4 days
  Refrigerated  4 days

Reference Values

Negative for extended-spectrum beta-lactamase-producing organisms


Positive for extended-spectrum beta-lactamase-producing organisms

Day(s) Performed

Monday through Sunday

Report Available

4 to 6 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87081-ESBL GNB surveillance culture

87077-Ident by MALDI-TOF mass spec (if appropriate)

87184-ESBL SUS test charge (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ESBLS ESBL GNB Surveillance Culture, F In Process


Result ID Test Result Name Result LOINC Value
ESBLS ESBL GNB Surveillance Culture, F In Process


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.