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Test ID HBGSN Hepatitis B Surface Antigen Screen, Serum

Ordering Guidance

This test should not be used to test symptomatic individuals (ie, diagnostic purposes) who may or may not have risk factors for hepatitis B virus infection. For testing such patients, order HBAG / Hepatitis B Surface Antigen, Serum.


This test should not be used to test or screen for chronic hepatitis B in pregnant individuals. For testing such patients, order HBAGP / Hepatitis B Surface Antigen Prenatal, Serum.


This test is not intended for testing cadaver or grossly hemolyzed specimens. For testing such patients, order HBGCD / Hepatitis B Surface Antigen for Cadaveric or Hemolyzed Specimens, Serum, which is US Food and Drug Administration approved for testing on these sources.

Additional Testing Requirements

Testing for acute hepatitis B virus infection (HBV) should also include HBIM / Hepatitis B Core Antibody, IgM, Serum, as during the acute HBV infection "window period," Hepatitis B surface (HBs) antigen and HBs antibody may not be detected.

Necessary Information

1. Date of collection is required.

2. Indicate if specimens are from autopsy/cadaver or hemolyzed sources so that the proper US Food and Drug Administration licensed assay can be performed.

Specimen Required

Collection Container/Tube: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Collection Instructions:

1. Centrifuge blood collection tube per collection tube manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).

2. Aliquot serum into plastic vial.

Useful For

Diagnosis of acute, recent, or chronic hepatitis B viral (HBV) infection


Determination of chronic HBV infection status


This test is not offered as a screening or confirmatory test for blood donor specimens.


This test is not useful for diagnosis of hepatitis B during the “window period" of acute HBV infection (ie, after disappearance of hepatitis B surface antigen and prior to appearance of hepatitis B surface antibody).

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
HBGSC HBs Antigen Screen Confirmation, S No No

Testing Algorithm

If hepatitis B surface antigen (HBsAg) screen is reactive with signal-to-cutoff ratio in the range of 1.00 to 100.0 then HBsAg confirmation will be performed at an additional charge.


The following algorithms are available:

-Hepatitis B: Testing Algorithm for Screening, Diagnosis, and Management

-HBV Infection-Monitoring Before and After Liver Transplantation

Method Name

Chemiluminescence Immunoassay

Reporting Name

HBs Antigen Scrn, S

Specimen Type

Serum SST

Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum SST Frozen (preferred) 28 days
  Refrigerated  7 days
  Ambient  24 hours

Reference Values


See Viral Hepatitis Serologic Profiles

Day(s) Performed

Monday through Saturday

Report Available

Same day/1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


G0499 (if appropriate)

87341 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HBGSN HBs Antigen Scrn, S 5196-1


Result ID Test Result Name Result LOINC Value
HBAGS HBs Antigen Scrn, S 5196-1


If not ordering electronically, complete, print, and send Gastroenterology and Hepatology Client Test Request (T728) with the specimen.