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Test ID MBLF Mannan Binding Lectin Complement Pathway, Functional, Serum

Ordering Guidance

The most appropriate primary assays to use as screening methods for complement deficiencies are COM / Complement, Total, Serum and AH50 / Alternative Complement Pathway, Functional, Serum. Abnormal results in one or the other, none, or both assays will help direct further testing. If total complement and AH50 are both normal but the suspicion of a complement deficiency remains, this test for the lectin pathway function is recommended. After the initial 3 pathways of complement functional status are evaluated, the analysis of individual components and regulators may uncover functional or quantitative defects in certain components.


This test is rarely useful when ordered in isolation.


As the heat-labile fraction of the immune system, complement activation in vitro has been a challenge for researchers and scientists. Because of the thermal instability of complement proteins, it is often necessary to repeat testing to rule out a possible pre-analytical issue with a sample type, real complement consumption, or dysregulation.

Specimen Required

Patient Preparation:

1. If plasma exchange has been performed, wait 48 hours before collecting the specimen.

2. Fasting is preferred.

Collection Container/Tube: Red top (serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Immediately after specimen collection, place the tube on wet ice.

2. Centrifuge at 4° C and aliquot serum into plastic vial.

3. Freeze specimen within 30 minutes.

Useful For

Investigating recurrent meningococcal disease in young children


Investigating recurrent or severe infections in adults


Investigating glomerular kidney diseases


Additionally, deficiencies or dysregulation within the complement system may be identified in patients when this test is used in combination with related tests.

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

MBL Complement Path, Func, S

Specimen Type

Serum Red

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Frozen 14 days

Reference Values


Day(s) Performed


Report Available

2 to 8 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
MBLF MBL Complement Path, Func, S 74522-4


Result ID Test Result Name Result LOINC Value
MBLF MBL Complement Path, Func, S 74522-4