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Test ID NMS1 Necrotizing Myopathy Evaluation, Serum

Necessary Information

Provide the following information:

-Relevant clinical information

-Ordering provider name, phone number, mailing address, and e-mail address

Specimen Required

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 3 mL

Collection Instructions: Centrifuge within 2 hours of collection and aliquot serum into a plastic vial.

Useful For

Evaluating patients with suspected necrotizing autoimmune myopathy

Profile Information

Test ID Reporting Name Available Separately Always Performed
NSI1 Necrotizing Myopathy Interp, S No Yes
HMGCR HMG-CoA Reductase Ab, S Yes Yes
SRPIS SRP IFA Screen, S No Yes

Testing Algorithm

This focused algorithmic test is designed to achieve high sensitivity for identification of antibodies specific for necrotizing autoimmune myopathy (HMGCOA-IgG and SRP-IgG). This test is unique in the market by having an initial screen for signal recognition particle (SRP) antibodies performed using tissue indirect immunofluorescence, which increases clinical sensitivity as compared to SRP immunoblot methodologies.


If indirect immunofluorescence assay (IFA) pattern suggests signal recognition particle (SRP) antibody, SRP IFA titer and SRP54 immunoblot are performed at an additional charge.


For more information, see Necrotizing Myopathy Evaluation Algorithm

Method Name

SRPIS, SRPTS: Indirect Immunofluorescence Assay (IFA)

SRPBS: Immunoblot

HMGCR: Chemiluminescent Assay (CIA)

NSI1: Medical Interpretation

Reporting Name

Necrotizing Myopathy Evaluation, S

Specimen Type


Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  72 hours

Reference Values

3-Hydroxy-3-Methylglutaryl Coenzyme-A (HMG-CoA) Reductase:

<20.0 CU


Signal Recognition Particle Antibody Screen:



Signal Recognition Particle Antibody:



Signal Recognition Particle Antibody, Titer:


Day(s) Performed

Tuesday, Thursday


Report Available

10 to 14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information



86256 (if appropriate)

84182 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
NMS1 Necrotizing Myopathy Evaluation, S 97561-5


Result ID Test Result Name Result LOINC Value
607414 HMG-CoA Reductase Ab, S 93493-5
603543 Necrotizing Myopathy Interp, S 69048-7
603540 SRP IFA Screen, S 97562-3

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
SRPBS SRP Immunoblot, S No No


If not ordering electronically, complete, print, and send a Neurology Specialty Testing Client Test Request (T732) with the specimen.