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HelpTest ID OVSRP Oxacillin-Hydrolyzing Beta-Lactamase (blaOXA-48-like) and Verona Integron-Encoded Metallo-Beta-Lactamase (blaVIM) DNA Surveillance, PCR, Varies
Useful For
Identifying carriers of Ggram-negative bacilli harboring oxacillin-hydrolyzing beta-lactamase (OXA-48-like) or Verona integron-encoded metallo-beta-lactamase (VIM) genes
Method Name
Real-Time Polymerase Chain Reaction (PCR) using LightCycler and Fluorescent Resonance Energy Transfer (FRET)
Reporting Name
OXA-48 and VIM Surveillance PCRSpecimen Type
VariesOrdering Guidance
This assay is for surveillance testing on perirectal/rectal/perianal/anal swabs or fecal specimens only. If testing isolates from culture, request OXVRP / Oxacillin-Hydrolyzing Beta-Lactamase (blaOXA-48-like) and Verona integron-encoded metallo-beta-lactamase (blaVIM) in Gram-Negative Bacilli, Molecular Detection, PCR, Varies.
Other mechanisms of carbapenem resistance, including other carbapenemase-types, porin mutations, or hyper-expression of drug efflux pumps may result in carbapenem resistance. These are not detected by this assay. If testing for Klebsiella pneumoniae carbapenemase (KPC) or New Delhi metallo-beta-lactamase (NDM) is desired, order KNSRP / Klebsiella pneumoniae Carbapenemase (blaKPC) and New Delhi Metallo-beta-lactamase (blaNDM) Surveillance, PCR, Varies.
Additional Testing Requirements
If testing for Klebsiella pneumoniae carbapenemase (KPC) or New Delhi metallo-beta-lactamase (NDM) is also needed; also order KNSRP / Klebsiella pneumoniae Carbapenemase (blaKPC) and New Delhi Metallo-beta-lactamase (blaNDM) Surveillance, PCR, Varies.
Necessary Information
Specimen source is required.
Specimen Required
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by oxacillin-hydrolyzing beta-lactamase (blaOXA-48-like) or Verona integron-encoded metallo-beta-lactamase (blaVIM) DNA is unlikely.
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Perianal, perirectal, rectal, anal
Supplies: Culturette (BBL Culture Swab) (T092)
Container/Tube: Culture transport swab (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)
Specimen Volume: Swab
Specimen Stability Information: Refrigerated (preferred)/Frozen
Acceptable:
Supplies: Culture and Sensitivity Stool Transport Vial (T058)
Specimen Type: Preserved feces
Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S vial)
Specimen Volume: Representative portion of feces
Collection Instructions:
1. Collect fresh fecal specimen and submit 1 gram or 5 mL in container with transport medium.
2. Place feces in preservative within 2 hours of collection.
Specimen Stability Information: Ambient (preferred)/Refrigerated
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Varies | ||
Reference Values
Negative
Reference values apply to all ages.
Day(s) Performed
Monday through Friday
Report Available
1 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87798 x 2
87999 (if appropriate for government payers)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| OVSRP | OXA-48 and VIM Surveillance PCR | 85502-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| OVSRC | Specimen Source | 31208-2 |
| 41746 | OXA-48-like PCR | 85827-4 |
| 41747 | VIM PCR | 85501-5 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.