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HelpTest ID OXVRP Oxacillin-Hydrolyzing Beta-Lactamase (blaOXA-48-like) and Verona Integron-Encoded Metallo-Beta-Lactamase (blaVIM) in Gram-Negative Bacilli, Molecular Detection, PCR, Varies
Infectious
Useful For
Assessing pure isolates of gram-negative bacilli for mechanism of carbapenem resistance
Special Instructions
Method Name
Real-Time Polymerase Chain Reaction (PCR) using LightCycler and Fluorescent Resonance Energy Transfer (FRET)
Reporting Name
OXA-48 and VIM PCRSpecimen Type
VariesOrdering Guidance
This assay should be used for testing of isolates of gram-negative bacilli. If testing directly from rectal swabs, perirectal swabs, anal swabs, perianal swabs, or stool is desired, order OVSRP / Oxacillin-Hydrolyzing Beta-Lactamase (blaOXA-48-like) and Verona Integron-Encoded Metallo-Beta-Lactamase (blaVIM) DNA Surveillance, PCR, Varies
Other mechanisms of carbapenem resistance, including other carbapenemase-types, porin mutations, or hyperexpression of drug efflux pumps may result in carbapenem resistance. These are not detected by this assay. If testing for Klebsiella pneumoniae carbapenemase (KPC) or New Delhi metallo-beta-lactamase (NDM) is desired, order KPNRP / KPC (blaKPC) and NDM (blaNDM) in Gram-Negative Bacilli, Molecular Detection, PCR.
Additional Testing Requirements
1. Organism identification must be provided. If organism identification is unknown, concomitantly order IDENT / Organism Referred for Identification, Aerobic Bacteria.
2. If susceptibility testing is needed; also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, Varies.
3. If testing for Klebsiella pneumoniae carbapenemase or New Delhi metallo-beta-lactamase is also needed; also order KPNRP / KPC (blaKPC) and NDM (blaNDM) in Gram-Negative Bacilli, Molecular Detection, PCR.
Shipping Instructions
1. For shipping information see Infectious Specimen Shipping Guidelines .
2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance, if appropriate.
Necessary Information
Organism identification and specimen source are required.
Specimen Required
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by oxacillin-hydrolyzing beta-lactamase (OXA-48-like) or Verona integron-encoded metallo-beta-lactamase (VIM) DNA is unlikely.
Supplies: Infectious Container, Large (T146)
Container/Tube: Slant
Specimen Volume: Isolate
Collection Instructions:
1. Perform isolation of infecting bacteria.
2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Reference Values
Not applicable
Day(s) Performed
Monday through Friday
Report Available
2 to 5 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87150 x 2
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| OXVRP | OXA-48 and VIM PCR | 85502-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| OXORG | Organism Identified by Client | 43409-2 |
| OXSRC | Specimen Source | 31208-2 |
| 41743 | OXA-48-like PCR | 85503-1 |
| 41744 | VIM PCR | 85501-5 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.