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Test ID PEISO Protein Electrophoresis and Isotype, Serum

Ordering Guidance

To monitor a patient with an established diagnosis of a monoclonal gammopathy, order TMOGA / Monoclonal Gammopathy, Monitoring, Serum.


Protein electrophoresis alone is not considered an adequate screen for monoclonal gammopathies. When screening a patient or establishing a first-time diagnosis for a monoclonal gammopathy, consider ordering DMOGA / Monoclonal Gammopathy, Diagnostic, Serum instead, which includes free light chain analysis.

Specimen Required

Patient Preparation: Fasting (12 hour) preferred but not required

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Renal Diagnostics Test Request (T830)

-General Request (T239)

-Hematopathology/Cytogenetics Test Request (T726)

Useful For

Diagnosing monoclonal gammopathies, when used in conjunction with locally performed serum free light chain studies

Profile Information

Test ID Reporting Name Available Separately Always Performed
TMAB Therapeutic Antibody Administered? No Yes
TPE Total Protein Yes, (Order TP) Yes
SPE Protein Electrophoresis No Yes
MPTS M-protein Isotype MALDI-TOF MS, S Yes, (Order MALD) Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IFXED Immunofixation Delta and Epsilon, S Yes No

Testing Algorithm

This test includes total protein, serum protein electrophoresis, and heavy and light chain typing (kappa and lambda).


If a light chain is identified without a corresponding heavy chain during initial testing, immunofixation with IgD and IgE antisera will be performed at an additional charge.


The following algorithms are available:

-Amyloidosis: Laboratory Approach to Diagnosis

-Multiple Myeloma: Laboratory Screening

Method Name

TMAB: Patient Information

TPE: Colorimetric, Biuret

SPE: Agarose Gel Electrophoresis

MPTS: Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)

Reporting Name

Prot Electrophoresis and Isotype, S

Specimen Type


Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days
  Ambient  7 days

Reference Values


≥1 year: 6.3-7.9 g/dL

Reference values have not been established for patients that are younger than 12 months of age.



Albumin: 3.4-4.7 g/dL

Alpha-1-globulin: 0.1-0.3 g/dL

Alpha-2-globulin: 0.6-1.0 g/dL

Beta-globulin: 0.7-1.2 g/dL

Gamma-globulin: 0.6-1.6 g/dL

An interpretive comment is provided with the report.

Reference values have not been established for patients that are younger than 16 years of age.



No monoclonal protein detected




Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information




86334 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PEISO Prot Electrophoresis and Isotype, S 90991-1


Result ID Test Result Name Result LOINC Value
TPE Total Protein 2885-2
602837 Albumin 2862-1
65198 M-protein Isotype MALDI-TOF MS 90990-3
TMAB Therapeutic Antibody Administered? 98855-0
606976 Flag, M-protein Isotype 94400-9
602838 Alpha-1 Globulin 2865-4
602839 Alpha-2 Globulin 2868-8
602840 Beta-Globulin 2871-2
602841 Gamma-Globulin 2874-6
602842 A/G Ratio 44429-9
602843 M spike 51435-6
602844 M spike 35559-4
602836 Impression 49296-7

Day(s) Performed

Monday through Friday

Report Available

2 to 5 days