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Test ID RPRT3 Rapid Plasma Reagin (RPR) with Reflex to Treponema pallidum Particle Agglutination, Serum

Ordering Guidance

This assay should only be used following an initial reactive treponemal antibody assay result as part of the reverse syphilis testing algorithm.

Specimen Required

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.3 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.

Useful For

Diagnosing syphilis

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RPRT4 RPR, Titer, S No No
RTPPA Syphilis Ab, TP-PA, S Yes, (Order TPPA) No

Testing Algorithm

If this test is positive, then the rapid plasma reagin titer will be performed at an additional charge.


If this test is negative, then the Treponema pallidum particle agglutination test will be performed at an additional charge.


For more information see Syphilis Serology Algorithm.

Special Instructions

Method Name

RPRT3, RPRT4: Flocculation/Agglutination

RTPPA: Particle Agglutination

Reporting Name

RPR w/ Reflex to TP-PA, S

Specimen Type


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reference Values


Reference values apply to all ages.

Day(s) Performed

Monday through Saturday

Report Available

Same day/1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


86593- (if appropriate)

86780- (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
RPRT3 RPR w/ Reflex to TP-PA, S 20507-0


Result ID Test Result Name Result LOINC Value
616970 RPR w/ Reflex to TP-PA, S 20507-0