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Test ID SRAU Serotonin Release Assay, Unfractionated Heparin, Mass Spectrometry, Serum

Shipping Instructions

Specimen should be frozen if shipped or stored for longer than 48 hours.

Specimen Required

Patient Preparation:

1. Specimen should be collected from a fasting (preferred, but not required) patient during an episode of suspected heparin induced thrombocytopenia.

2. Patient should not be on ticagrelor (Brilinta) as this may interfere with the assay, yielding a false-negative result.

Supplies: Sarstedt 5 mL Aliquot Tube (T914)

Collection Container/Tube: Red top (serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. After collection, specimen should sit at ambient temperature for a minimum of 1 hour in order to clot completely.

2. Centrifuge and aliquot serum into a plastic vial.

Specimen Stability Information: Frozen (preferred) 2 years/Refrigerate 7 days

Useful For

Detecting heparin-dependent platelet activating antibodies implicated in the pathogenesis of heparin-induced thrombocytopenia

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Serotonin Release Assay, UFH, MS, S

Specimen Type

Serum Red

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Frozen (preferred)
  Refrigerated  7 days

Reference Values

Low Heparin Serotonin Release:


High Heparin Serotonin Release:


Serotonin Release Assay Result:


Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
SRAU Serotonin Release Assay, UFH, MS, S 50736-8


Result ID Test Result Name Result LOINC Value
616230 Low Heparin Serotonin Release 50728-5
616231 High Heparin Serotonin Release 50727-7
616232 Serotonin Release Assay Result 66488-8
616233 Interpretation 50733-5
616234 Comment 77202-0
616235 Disclaimer 62364-5


If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.