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Test ID TALMF T-Cell Acute Lymphoblastic Leukemia/Lymphoma (ALL), Specified FISH, Varies

Ordering Guidance

This test is intended for instances when limited T-cell acute lymphoblastic leukemia (ALL) fluorescence in situ hybridization (FISH) probes are needed. The FISH probes to be analyzed must be specified on the request, otherwise test processing may be delayed in order to determine intended analysis.

-For an adult patient, if the entire T-cell ALL FISH panel is preferred, order TALAF / T-Cell Acute Lymphoblastic Leukemia/Lymphoma (ALL), FISH, Adult, Varies.

-For a pediatric patient, if the entire T-cell ALL FISH panel is desired, order TALPF / T-Cell Acute Lymphoblastic Leukemia/Lymphoma (ALL), FISH, Pediatric, Varies.

-If this test is ordered and the laboratory is informed that the patient is on a Children's Oncology Group (COG) protocol, this test will be canceled and automatically reordered by the laboratory as COGTF / T-Cell Acute Lymphoblastic Leukemia/Lymphoma (ALL), Children's Oncology Group Enrollment Testing, FISH, Varies.


If the patient clinically relapses, a conventional chromosome study is useful to identify cytogenetic changes in the neoplastic clone or the possible emergence of a new therapy-related myeloid clone.


At diagnosis, conventional cytogenetic studies (CHRBM / Chromosome Analysis, Hematologic Disorders, Bone Marrow) and a complete TALAF / T-Cell Acute Lymphoblastic Leukemia/Lymphoma (ALL), FISH, Adult, Varies or TALPF / T-Cell Acute Lymphoblastic Leukemia/Lymphoma (ALL), FISH, Pediatric, Varies should be performed, depending on patient's age.


For patients with T-cell lymphoma, order TLPDF / T-Cell Lymphoma, Diagnostic FISH, Varies.


For testing paraffin-embedded tissue samples from patients with T-lymphoblastic lymphoma, order TLBLF / T-Cell Lymphoblastic Leukemia/Lymphoma, FISH, Tissue. If a paraffin-embedded tissue sample is submitted for this test, this test will be canceled and TLBLF will be added and performed as the appropriate test.

Shipping Instructions

Advise Express Mail or equivalent if not on courier service.

Necessary Information

1. A list of probes requested for analysis is required. Probes available for this test are listed in the Testing Algorithm section.

2. A reason for testing and a flow cytometry and/or a bone marrow pathology report should be submitted with each specimen. The laboratory will not reject testing if this information is not provided; however, appropriate testing and/or interpretation may be compromised or delayed in some instances. If not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.

Specimen Required

Submit only 1 of the following specimens:



Specimen Type: Bone marrow


Preferred: Yellow top (ACD)

Acceptable: Green top (heparin) or lavender top (EDTA)

Specimen Volume: 2 to 3 mL

Collection Instructions:

1. It is preferable to send the first aspirate from the bone marrow collection.

2. Invert several times to mix bone marrow.

3. Send bone marrow specimen in original tube. Do not aliquot.



Specimen Type: Blood


Preferred: Yellow top (ACD)

Acceptable: Green top (heparin) or lavender top (EDTA)

Specimen Volume: 6 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in original tube. Do not aliquot.

Useful For

Detecting a neoplastic clone associated with the common chromosome abnormalities and classic rearrangements observed in patients with T-cell acute lymphoblastic leukemia (T-ALL) using client specified probes


An adjunct to conventional chromosome studies in patients with T-ALL


Evaluating specimens in which standard cytogenetic analysis is unsuccessful


Identifying and tracking known chromosome abnormalities in patients with T-ALL and monitoring response to therapy

Testing Algorithm

This test includes a charge for the probe application, analysis, and professional interpretation of results for 1 probe set (2 individual fluorescence in situ hybridization (FISH) probes). Additional charges will be incurred for all reflex or additional probe sets performed.


If the patient is being treated for known abnormalities, indicate the abnormality and which probes should be used.


When specified, any of the following probes will be performed:

1p33 rearrangement, TAL1/STIL

t(5;14), TLX3/BCL11B

5q32 rearrangement, PDGFRB break-apart

7q34 rearrangement, TRB break-apart

t(6;7)(q23;q34) MYB/TRB

t(7;10)(q34;q24) TRB/TLX1

t(7;11)(q34;p15) TRB/LMO1

t(7;11)(q34;p13) TRB/LMO2

+9/9p-, CDKN2A/D9Z1

9p24.1 rearrangement, JAK2 break-apart

t(9;22) or ABL1 amplification, ABL1/BCR

9q34 rearrangement, ABL1 break-apart

t(10;11), MLLT10/PICALM

11q23 rearrangement, MLL (KMT2A) break-apart

t(4;11)(q21;q23) AFF1/MLL

t(6;11)(q27;q23) MLLT4(AFDN)/MLL

t(9;11)(p22;q23) MLLT3/MLL

t(10;11)(p12;q23) MLLT10/MLL

t(11;19)(q23;p13.1) MLL/ELL

t(11;19)(q23;p13.3) MLL/MLLT1

14q11.2 rearrangement, TRAD break-apart

t(8;14)(q24.1;q11.2) MYC/TRAD

t(10;14)(q24;q11.2) TLX1/TRAD

t(11;14)(p15;q11.2) LMO1/TRAD

t(11;14)(p13;q11.2) LMO2/TRAD

-17/17p-, TP53/D17Z1

Method Name

Fluorescence In Situ Hybridization (FISH)

Reporting Name

ALL (T-cell), Specified FISH

Specimen Type


Specimen Minimum Volume

Blood: 2 mL
Bone Marrow: 1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient (preferred)

Reference Values

An interpretive report will be provided.

Day(s) Performed

Monday through Friday

Report Available

7 to 10 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88271x2, 88275x1, 88291x1-FISH Probe, Analysis, Interpretation; 1 probe set

88271x2, 88275x1 - FISH Probe, Analysis; each additional probe set (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TALMF ALL (T-cell), Specified FISH In Process


Result ID Test Result Name Result LOINC Value
614325 Result Summary 50397-9
614326 Interpretation 69965-2
614327 Result Table 93356-4
614328 Result 62356-1
GC134 Reason for Referral 42349-1
GC135 Probes Requested 78040-3
GC136 Specimen 31208-2
614329 Source 31208-2
614330 Method 85069-3
614331 Additional Information 48767-8
614332 Disclaimer 62364-5
614333 Released By 18771-6

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
TALMB Probe, Each Additional (TALMF) No, (Bill Only) No


If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.