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Test ID TBIDS Culture Referred for Identification, Mycobacterium and Nocardia with Antimicrobial Susceptibility Testing, Varies


Shipping Instructions

1. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.

Necessary Information

1. Specimen source is required.

2. Isolate description is required: Gram stain reaction, morphology, tests performed.

Specimen Required

Specimen Type: Organism in pure culture

Supplies: Infectious Container, Large (T146)

Container/Tube: Middlebrook (7H10 or 7H11) or Lowenstein-Jensen medium slant or in broth (eg, Mycobacteria Growth Indicator Tube [7H9] broth)

Specimen Volume: Isolate

Collection Instructions: Organism must be in pure culture, actively growing. Do not submit mixed cultures.

Useful For

Rapid identification to the species level and susceptibility testing for Mycobacterium species, Nocardia species, and other aerobic actinomycete genera and species from pure culture isolates

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RMALM Id MALDI-TOF Mass Spec AFB No, (Bill Only) No
RTBSP Id, Mtb Speciation, PCR No, (Bill Only) No
TBMP Mycobacteria Probe Ident No, (Bill Only) No
TBPB Mycobacteria Probe Ident Broth No, (Bill Only) No
ISMY ID by 16S Sequencing No, (Bill Only) No
SRG Susceptibility Rapid Grower No, (Bill Only) No
RSLG Susceptibility Slow Grower No, (Bill Only) No
SSNS Susceptibility Nocardia species No, (Bill Only) No
STV1 Susceptibility, Mtb Complex, Broth No, (Bill Only) No
STV2 Susceptibility, Mtb Cx, 2nd Line No, (Bill Only) No
STVP Susceptibility, Mtb Complex, PZA No, (Bill Only) No
MTBVP Mtb PZA Confirmation, pnc A Sequence No, (Bill Only) No
MIC Susceptibility, MIC No, (Bill Only) No
LCTB Id, MTB complex Rapid PCR No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex antimicrobial susceptibility test will be performed at an additional charge. All mycobacteria and Nocardia (including aerobic actinomycetes) submitted will be identified and billed as appropriate.


Nucleic acid probes used for identification, when applicable, include those for Mycobacterium avium-intracellulare complex, Mycobacterium gordonae, and Mycobacterium tuberculosis complex. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF MS) and/or 16S rDNA sequencing is used for identification, when applicable, for slowly and rapidly growing Mycobacterium species and aerobic actinomycetes (including Nocardia species and Streptomyces species). The M tuberculosis complex can be further identified to the species level upon request, using a separate rapid PCR test. Minimum inhibitory concentration (MIC) determination by either the microtiter broth dilution method or critical concentration testing by broth dilution will be automatically performed as appropriate after species identification.


See Culture Referred for Identification and Susceptibility for Mycobacterium and Nocardia Algorithm in Special Instructions.

Method Name

Nucleic Acid Probe/16S rDNA Sequencing/Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI TOF MS)/Rapid Polymerase Chain Reaction (PCR)

Reporting Name

Mycobacteria Culture Refer ID+Susc

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient (preferred)

Reference Values

Not applicable

Day(s) Performed

Monday through Sunday

Report Available

60 to 70 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

Culture Referred for Identification, Mycobacterium

87118-Identificaiton of mycobacteria

87158-Identification of mycobacteria by other methods (if appropriate)

87118 -Id MALDI-TOF Mass Spec AFB (if appropriate)

87153-Mycobacteria Identification by Sequencing (if appropriate)

87150-Mycobacteria Probe Ident, Solid (if appropriate)

87150-Mycobacteria Probe Ident, Broth (if appropriate)

87150-Id, Mtb Speciation, PCR (if appropriate)

87186-Susceptibility Rapid Grower (if appropriate)

87186-Susceptibility Slow Grower (if appropriate)

87186-Susceptibility Nocardia species (if appropriate)

87188 x 3-Antimicrobial Susceptibility, Mycobacterium tuberculosis Complex, Broth Method (if appropriate)

87186-Suscetpbility, Mtb Cx, 2nd Line (if appropriate)

87188-Susceptibility, Mycobacterium tuberculosis Complex, Pyrazinamide (if appropriate)

87153-Mtb PZA Confirmation, pncA Sequencing (if appropriate)

87150- Id, MTB complex Rapid PCR (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TBIDS Mycobacteria Culture Refer ID+Susc 543-9


Result ID Test Result Name Result LOINC Value
TBIDS Mycobacteria Culture Refer ID+Susc 543-9


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.