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HelpTest ID TLBLF T-Lymphoblastic Leukemia/Lymphoma, FISH, Tissue
Shipping Instructions
Advise Express Mail or equivalent if not on courier service.
Necessary Information
A reason for referral and pathology report are required in order for testing to be performed. Send information with specimen. Acceptable pathology reports include working drafts, preliminary pathology or surgical pathology reports.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Tissue
Container/Tube: Formalin-fixed, paraffin-embedded tumor tissue block
Specimen Type: Slides
Specimen Volume: 19 Consecutive, unstained, 5 micron-thick sections placed on positively charged slides and 1 hematoxylin and eosin-stained slide
Useful For
Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with T-cell lymphoblastic leukemia or lymphoma
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| _I300 | Interphases, >=100 | No | No |
| _I099 | Interphases, 25-99 | No | No |
| _IL25 | Interphases, <25 | No | No |
| _PB03 | Probe, +3 | No | No |
| _PB02 | Probe, +2 | No | No |
| _PADD | Probe, +1 | No | No |
| _PBCT | Probe, +2 | No | No |
Testing Algorithm
This test may be ordered in 2 distinct ways allowing different combinations of probes to be utilized based on the clinical question, including the standard (diagnostic) TLBL FISH panel and the individual TLBL FISH probes (per client request).
The specific TLBL FISH panel or probes requested must be noted on the request form or in the reason for referral. If no specific panel or FISH probes are indicated, the standard (diagnostic) panel will be performed.
The standard (diagnostic) panel includes testing for the following abnormalities, using the FISH probes listed:
-1p33 rearrangements, TAL1/STIL(SIL)
-t(5;14)(q35;q32), TLX3(HOX11L2)/BCL11B
-7q34 rearrangements, TRB(TCR beta)
-9p-, CDKN2A(p16)/D9Z1
-t(9;22)(q34;q11.2), ABL1 amplification, ABL1(ABL)/BCR
-t(10;11)(p12;q14), MLLT10(AF10)/PICALM
-11q23 rearrangements, MLL
-14q11.2 rearrangements, TRAD (TCR alpha delta)
-17p-, TP53/D17Z1
When an MLL rearrangement is identified, reflex testing will be performed with 1 or more dual-fusion probe sets (D-FISH) in an attempt to identify the translocation partner. Probes include:
-t(4;11)(q21;q23), AFF1(AF4)/MLL
-t(6;11)(q27;q23), MLLT4(AF6)/MLL
-t(9;11)(p22;q23), MLLT3(AF9)/MLL
-t(10;11)(p12;q23), MLLT10(AF10)/MLL
-t(11;19)(q23;p13.1), MLL/ELL
-t(11;19)(q23;p13.3), MLL/MLLT1(ENL)
When an TRB(TCR beta) rearrangement is identified, reflex testing will be performed with a dual-fusion probe sets
(D-FISH) in an attempt to identify the translocation partner.
-t(7;10)(q34;q24), TRB(TCR beta)/TLX1(HOX11)
When an TRAD(TCR alpha delta) rearrangement is identified, reflex testing will be performed with a dual-fusion probe sets
(D-FISH) in an attempt to identify the translocation partner.
-t(10;14)(q24;q11.2), TLX1(HOX11)/TRAD(TCR alpha delta)
This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.
Method Name
Fluorescence In Situ Hybridization (FISH)
Reporting Name
T-Lymphoblastic Leuk/Lymph, FISH,TsSpecimen Type
TissueSpecimen Minimum Volume
Formalin-fixed paraffin-embedded tissue block or for each probe set ordered, 9 unstained consecutive tissue sections cut at 5 microns and placed on positively charged microscope slides. Include 1 hematoxylin and eosin (H and E) stained slide.
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Tissue | Ambient (preferred) | ||
| Refrigerated | |||
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Friday
Report Available
7 to 10 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88271 x 2, 88291-DNA probe, each (first probe set), Interpretation and report
88271 x 2-DNA probe, each; each additional probe set (if appropriate)
88271-DNA probe, each; coverage for sets containing 3 probes (if appropriate)
88271 x 2-DNA probe, each; coverage for sets containing 4 probes (if appropriate)
88271 x 3-DNA probe, each; coverage for sets containing 5 probes (if appropriate)
88274 w/modifier 52-Interphase in situ hybridization, <25 cells, each probe set (if appropriate)
88274-Interphase in situ hybridization, 25 to 99 cells, each probe set (if appropriate)
88275-Interphase in situ hybridization, 100 to 300 cells, each probe set (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TLBLF | T-Lymphoblastic Leuk/Lymph, FISH,Ts | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 113467 | Result Summary | 50397-9 |
| 113468 | Interpretation | 69965-2 |
| 113626 | Result Table | 93356-4 |
| 113469 | Result | 62356-1 |
| GC005 | Reason for Referral | 42349-1 |
| 113470 | Specimen | 31208-2 |
| 113471 | Source | 31208-2 |
| 113472 | Tissue ID | 80398-1 |
| 113473 | Method | 85069-3 |
| 113474 | Additional Information | 48767-8 |
| 113475 | Disclaimer | 62364-5 |
| 113476 | Released By | 18771-6 |
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen: