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Test ID TLYM T-Cell Lymphoma, FISH, Tissue

Ordering Guidance

This test does not include a pathology consultation. If a pathology consultation is requested, order PATHC / Pathology Consultation; the appropriate fluorescence in situ hybridization (FISH) test will be ordered and performed at an additional charge. Mayo Hematopathology Consultants are involved in both the pre-analytic (tissue adequacy and probe selection, when applicable) and post-analytic (interpretation of FISH results in context of specific case, when applicable) phases.


This assay detects chromosome abnormalities observed in paraffin-embedded tissue specimens of patients with T-cell lymphoma. For testing the blood and bone marrow from patients with T-cell lymphoma, see TLPDF / T-Cell Lymphoma, Diagnostic FISH, Varies or TLPMF / T-Cell Lymphoma, Specified FISH, Varies.

Shipping Instructions

Advise Express Mail or equivalent if not on courier service.

Necessary Information

1. A reason for testing and pathology report are required for testing to be performed. Send information with specimen. Acceptable pathology reports include working drafts, preliminary pathology or surgical pathology reports. The laboratory will not reject testing if a reason for testing is not provided; however appropriate testing and interpretation may be compromised or delayed. If not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.

2. If the patient is being tracked for known abnormalities, an indication of which probes should be used is required for testing to be performed. See Table in Clinical Information.

Specimen Required

Specimen Type: Tissue

Preferred: Tissue block

Collection Instructions: Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.

Additional Information:

1. Paraffin embedded specimens can be from any anatomic location (skin, soft tissue, lymph node, etc).

2. Bone specimens that have been decalcified will be attempted for testing, but the success rate is approximately 50%.


Acceptable: Tissue slides

Collection Instructions: For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides. Include 1 hematoxylin and eosin-stained slide for the entire test order.

Useful For

Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with various T-cell lymphomas


Tracking known chromosome abnormalities and response to therapy in patients with T-cell lymphomas


Providing potentially prognostic information in patients with documented systemic anaplastic lymphoma kinase-negative anaplastic large cell lymphoma


Supporting the diagnosis of peripheral T-cell lymphoma when coordinated with consultation by anatomic pathology

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
_PRAH Probe, Each Additional (TLYM) No, (Bill Only) No

Testing Algorithm

Depending on the lymphoma subtype suspected, the most appropriate probes to order are listed in the table Common Chromosome Abnormalities in T-cell Lymphomas in Clinical Information. The TBL1XR1/TP63 FISH probe will only be performed, at the laboratory’s discretion, to resolve or confirm TP63 rearrangement concerns.


If the patient is being tracked for known abnormalities, indicate which probes should be used.


A charge and CPT code is applied for each probe set hybridized, analyzed, and reported.


If, based on testing algorithms, results of the initial probe sets require reflex testing, complete results will be available within 10 days.

Method Name

Fluorescence In Situ Hybridization (FISH)

Reporting Name

T-cell Lymphoma, FISH, Tissue

Specimen Type


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Tissue Ambient (preferred)

Reference Values

An interpretive report will be provided.

Day(s) Performed

Monday through Friday

Report Available

7 to 10 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88377 (if 1 probe set)

88377 x 2 (if 2 probe sets)

88377 x 3 (if 3 probe sets)

88377 x 4 (if 4 probe sets)

88377 x 5 (if 5 probe sets)

88377 x 6 (if 6 probe sets)

88377 x 7 (if 7 probe sets)

88377 x 8 (if 8 probe sets)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TLYM T-cell Lymphoma, FISH, Tissue In Process


Result ID Test Result Name Result LOINC Value
603140 Result Summary 50397-9
603141 Interpretation 69965-2
603142 Result Table 93356-4
603143 Result 62356-1
GC040 Reason for Referral 42349-1
603144 Specimen 31208-2
603145 Source 31208-2
603146 Tissue ID 80398-1
603147 Method 85069-3
603148 Additional Information 48767-8
603149 Disclaimer 62364-5
603150 Released By 18771-6


If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.