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Test ID VITB3 Vitamin B3 and Metabolites, Plasma

Specimen Required

Patient Preparation: Fasting 4 to 8 hours preferred

Supplies: Sarstedt 5 mL Aliquot Tube (T914)

Collection Container/Tube:

Preferred: Lavender top (K2 EDTA)

Acceptable: K3 EDTA

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions: Centrifuge and aliquot plasma into a plastic vial within 2 hours of collection.

Useful For

Assisting in the diagnosis of suspected vitamin B3 deficiency or toxicity


May be useful in determining response to therapy

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Vitamin B3 and Metabolites, P

Specimen Type

Plasma EDTA

Specimen Minimum Volume

0.75 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  21 days

Reference Values

Nicotinic Acid (Niacin) Cutoff: <5.0 ng/mL

Nicotinamide: 5.0-48.0 ng/mL

Nicotinuric Acid Cutoff: <5.0 ng/mL

Day(s) Performed

Monday, Tuesday, Thursday, Friday

Report Available

2 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
VITB3 Vitamin B3 and Metabolites, P In Process


Result ID Test Result Name Result LOINC Value
609493 Nicotinic Acid (Niacin) 18244-4
609494 Nicotinamide 56961-6
609495 Nicotinuric Acid 72306-4